transcription 3 stat3 (Proteintech)

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Proteintech transcription 3 stat3

MFAP2 promotes epithelial–mesenchymal transition (EMT) through the <t>EGFR-AKT-STAT3</t> signaling pathway in colorectal cancer (CRC) cells. (A, B) The top 20 enrichment signaling pathways regulated by MFAP2 knockdown. (C, D) VEGFR2 signaling pathway was enriched in the MFAP2 knockdown cells, shown by Gene Set Enrichment Analysis (GSEA) and essential genes in this enrichment. (E) MFAP2 knockdown affected the EGFR-AKT-STAT3 axis.

Transcription 3 Stat3, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 967 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+stat3/STAT3+Antibody/pmc12914112-62-53-56
Average 96 stars, based on 967 article reviews

transcription 3 stat3 - by Bioz Stars, 2026-09

96/100 stars

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1) Product Images from "MFAP2 promotes metastasis and drug resistance by regulating epithelial-to-mesenchymal transition through EGFR signaling pathway in colorectal cancer cells"

Article Title: MFAP2 promotes metastasis and drug resistance by regulating epithelial-to-mesenchymal transition through EGFR signaling pathway in colorectal cancer cells

Journal: Genes & Diseases

doi: 10.1016/j.gendis.2025.101800

MFAP2 promotes epithelial–mesenchymal transition (EMT) through the EGFR-AKT-STAT3 signaling pathway in colorectal cancer (CRC) cells. (A, B) The top 20 enrichment signaling pathways regulated by MFAP2 knockdown. (C, D) VEGFR2 signaling pathway was enriched in the MFAP2 knockdown cells, shown by Gene Set Enrichment Analysis (GSEA) and essential genes in this enrichment. (E) MFAP2 knockdown affected the EGFR-AKT-STAT3 axis.

Figure Legend Snippet: MFAP2 promotes epithelial–mesenchymal transition (EMT) through the EGFR-AKT-STAT3 signaling pathway in colorectal cancer (CRC) cells. (A, B) The top 20 enrichment signaling pathways regulated by MFAP2 knockdown. (C, D) VEGFR2 signaling pathway was enriched in the MFAP2 knockdown cells, shown by Gene Set Enrichment Analysis (GSEA) and essential genes in this enrichment. (E) MFAP2 knockdown affected the EGFR-AKT-STAT3 axis.

Techniques Used: Protein-Protein interactions, Knockdown

Blocking Assay:

Article Title: Crocetin suppresses colorectal cancer progression by targeting TGM2 and inhibiting the JAK2/STAT3 pathway.
Article Snippet: Background Colorectal cancer (CRC) is a major health threat with limited therapies for advanced stages.. Crocetin, a natural compound from saffron, has broad anticancer potential, but its mechanisms in CRC are unclear.. Methods A CRC xenograft mouse model was established to evaluate the antitumor effect of crocetin.

Incubation:

Article Title: Smoking promotes colorectal cancer via the CKAP2L/AREG axis.
Article Snippet: .. After fragmentation, the lysate was incubated with 2 μg anti‐STAT3 (cat. no. 60199‐1‐Ig; Proteintech Group, Inc.) or 2 μg IgG (cat. no. 30000‐0‐AP; Proteintech Group, Inc.) at 4 ̊C overnight. .. Subsequently, 80 μl protein A/G beads (MedChemExpress) were incubated with the aforementioned antibody and lysate complex at 4 ̊C for 1 h and chromatin was eluted with elution buffer (1% SDS and 0.1 M NaHCO3) and was incubated at 65 ̊C for 4 h to reverse crosslinking.

Article Title: Extracellular vesicles derived from live or apoptotic mesenchymal stem cells: comparison of the effects of two extracellular vesicles on liver fibrosis.
Article Snippet: Subsequently, equal amounts of protein samples (30 μg) were loaded onto SDS-PAGE gels and transferred to nitrocellulose membranes (Bio-Rad Biotechnology, USA). .. The membranes were blocked in TBST buffer containing 2.5% skim milk for 30 min. Then, the membranes were incubated overnight at 4 °C with the following primary antibodies: anti-Caspase-3 (9662, Cell Signaling Technology, USA; diluted at 1:1000), anti-GM130 (ab52649, Abcam, UK; diluted at 1:1000), anti-Calnexin (10427-2-AP, Proteintech, USA; diluted at 1:1000), anti-CD81 (ab79559, Abcam, UK; diluted at 1:1000), anti-CD9 (ab236630, Abcam, UK; diluted at 1:1000), anti-Collagen I (ab270993, Abcam, UK; diluted at 1:1000), anti-αSMA (19245, Cell Signaling Technology, USA; diluted at 1:1000), anti-NOS2 (ab15323, Abcam, UK; diluted at 1:1000), antiARG-1 (ab124917, Abcam, UK; diluted at 1:1000), anti-STAT3 (10253-2-AP, Proteintech, USA; diluted at 1:1000), anti-STAT6 (9362, Cell Signaling Technology, USA; diluted at 1:1000), anti- NF-κB (8242, Cell Signaling Technology, USA; diluted at 1:1000), anti-AKT (4691, Cell Signaling Technology, USA; diluted at 1:1000), and anti-β-Actin (66009-1-Ig, Proteintech, USA; diluted at 1:5000). ..

Article Title: Phosphatidylserine Decarboxylase Promotes Ferroptosis Through STAT3/GPX4 Signaling in Gastric Cancer
Article Snippet: The lysate was centrifuged at 12,000× g at 4 ◦C for 20 min. A BCA protein determination kit (Beyotime, Shanghai, China) was used to measure the protein concentration. .. Proteins were separated by 10% SDS-PAGE and transferred to polyvinylidene difluoride membranes (Millipore, Burlington, MA, USA), which were blocked with 5% nonfat milk for 2 h. The membranes were incubated overnight at 4 ◦C with the following specific antibodies: anti-PISD (Santa Cruz Biotechnology, Dallas, TX, USA; CAT#sc-390070 and Proteintech, Wuhan, China; CAT# 68484-1-Ig), anti-STAT3 (Proteintech, Wuhan, China; CAT# 60199-1-Ig), anti-pSTAT3 (Proteintech, Wuhan, China; Cat No. 60479-1-Ig), anti-GPX4 (Zenoio, Chengdu, China; CAT# R24461), anti-GAPDH (Affinity Biosciences, Cincinnati, OH, USA; CAT#T0004), antiJAK2 (Servicebio, Wuhan, China; CAT#GB11325-50), and anti-pJAK2 (Servicebio, Wuhan, China; CAT#GB114585-100). .. After washing with TBST three times, the membranes were incubated with an HRP-conjugated secondary antibody at room temperature for 1 h. Finally, the membranes were washed and exposed to an ECL substrate (GE Healthcare, Shanghai, China).

Article Title: Smoking promotes colorectal cancer via the CKAP2L/AREG axis
Article Snippet: .. After fragmentation, the lysate was incubated with 2 μ g anti-STAT3 (cat. no. 60199-1-Ig; Proteintech Group, Inc.) or 2 μ g IgG (cat. no. 30000-0-AP; Proteintech Group, Inc.) at 4°C overnight. .. Subsequently, 80 μ l protein A/G beads (MedChemExpress) were incubated with the aforementioned antibody and lysate complex at 4°C for 1 h and chromatin was eluted with elution buffer (1% SDS and 0.1 M NaHCO 3 ) and was incubated at 65°C for 4 h to reverse crosslinking.

Article Title: Phosphatidylserine Decarboxylase Promotes Ferroptosis Through STAT3/GPX4 Signaling in Gastric Cancer
Article Snippet: The lysate was centrifuged at 12,000× g at 4 °C for 20 min. A BCA protein determination kit (Beyotime, Shanghai, China) was used to measure the protein concentration. .. Proteins were separated by 10% SDS-PAGE and transferred to polyvinylidene difluoride membranes (Millipore, Burlington, MA, USA), which were blocked with 5% nonfat milk for 2 h. The membranes were incubated overnight at 4 °C with the following specific antibodies: anti-PISD (Santa Cruz Biotechnology, Dallas, TX, USA; CAT# sc-390070 and Proteintech, Wuhan, China; CAT# 68484-1-Ig), anti-STAT3 (Proteintech, Wuhan, China; CAT# 60199-1-Ig), anti-pSTAT3 (Proteintech, Wuhan, China; Cat No. 60479-1-Ig), anti-GPX4 (Zenoio, Chengdu, China; CAT# R24461 ), anti-GAPDH (Affinity Biosciences, Cincinnati, OH, USA; CAT# T0004), anti-JAK2 (Servicebio, Wuhan, China; CAT# GB11325-50), and anti-pJAK2 (Servicebio, Wuhan, China; CAT# GB114585-100). .. After washing with TBST three times, the membranes were incubated with an HRP-conjugated secondary antibody at room temperature for 1 h. Finally, the membranes were washed and exposed to an ECL substrate (GE Healthcare, Shanghai, China).

SDS Page:

Article Title: Phosphatidylserine Decarboxylase Promotes Ferroptosis Through STAT3/GPX4 Signaling in Gastric Cancer
Article Snippet: The lysate was centrifuged at 12,000× g at 4 °C for 20 min. A BCA protein determination kit (Beyotime, Shanghai, China) was used to measure the protein concentration. .. Proteins were separated by 10% SDS-PAGE and transferred to polyvinylidene difluoride membranes (Millipore, Burlington, MA, USA), which were blocked with 5% nonfat milk for 2 h. The membranes were incubated overnight at 4 °C with the following specific antibodies: anti-PISD (Santa Cruz Biotechnology, Dallas, TX, USA; CAT# sc-390070 and Proteintech, Wuhan, China; CAT# 68484-1-Ig), anti-STAT3 (Proteintech, Wuhan, China; CAT# 60199-1-Ig), anti-pSTAT3 (Proteintech, Wuhan, China; Cat No. 60479-1-Ig), anti-GPX4 (Zenoio, Chengdu, China; CAT# R24461 ), anti-GAPDH (Affinity Biosciences, Cincinnati, OH, USA; CAT# T0004), anti-JAK2 (Servicebio, Wuhan, China; CAT# GB11325-50), and anti-pJAK2 (Servicebio, Wuhan, China; CAT# GB114585-100). .. After washing with TBST three times, the membranes were incubated with an HRP-conjugated secondary antibody at room temperature for 1 h. Finally, the membranes were washed and exposed to an ECL substrate (GE Healthcare, Shanghai, China).

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